Introducing Expanded MiDOG Testing
Now with Parasites, Biofilms/Toxin Detection + Longitudinal Reporting Request Expanded Testing InformationOrder Test Kits
What You Get from Expanded MiDOG Testing
MiDOG’s expanded testing enhances clinical decision-making by combining broad microbial detection with clinically relevant genomic insight, helping clinicians interpret results and determine clearer next steps in complex or unresolved cases.
Included:
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Broad microbial identification across all bacteria and fungi
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Detection of fastidious, slow-growing, or difficult-to-culture organisms
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Genomic markers associated with antimicrobial resistance (genotypic; supports stewardship discussions)
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Genomic indicators associated with pathogenic potential, providing added clinical context
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Interpretation support to help prioritize organisms based on clinical relevance
Optional add-on modules (when indicated): Parasites • Biofilm-Associated Markers • Toxin-Associated Markers
Broad Microbial Detection with Expanded Clinical Context
Supporting detection across tens of thousands of microbial organisms while providing additional insight into virulence factors associated with persistent infection.
Bacteria
Detects bacterial DNA across more than 64,800 bacterial organisms, supporting diagnosis of polymicrobial infections, chronic disease, and cases where traditional culture may miss clinically relevant pathogens. Results are paired with genotypic antimicrobial resistance markers to help guide targeted antimicrobial selection.
Fungi
Screens for more than 4,100 fungal organisms, helping clinicians identify fungal pathogens involved in dermatologic, respiratory, gastrointestinal, and systemic infections. DNA-based detection supports cases where fungal involvement is suspected but difficult to confirm using conventional diagnostic methods.
Parasites
Detects DNA from over 640 parasitic organisms, supporting workups for gastrointestinal disease, exposure risk cases, and patients with persistent or unexplained clinical signs. Expanded parasite detection helps clinicians evaluate infections that may not be captured through routine diagnostic testing.
Toxins / Biofilm Markers
Screens for toxin-associated targets and biofilm-related markers that may contribute to persistent or treatment-resistant infections. These markers provide additional clinical context when chronicity, recurrence, or treatment failure raises concern for microbial virulence or biofilm-associated persistence.
When Should I Add Expanded Testing or Advanced Add-Ons?
Consider expanded testing or optional add-on modules when additional diagnostic depth is needed, particularly in cases where initial testing or traditional diagnostics do not provide a clear clinical direction.
Best-fit scenarios:
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Recurrent or non-resolving disease despite treatment
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Culture-negative, inconclusive, or conflicting diagnostic results
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Suspected polymicrobial infection or mixed etiologies
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Cases where biofilm involvement or toxin production may influence treatment response
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High-risk patients (immunocompromised, exotic or zoological species, or high-value animals)
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Biosecurity or collection medicine situations (group housing, outbreak investigation, entry/exit screening support)
Quick cue: If the case is not responding as expected or additional context is needed to guide next-step decisions, expanded testing and targeted add-on insights may help clarify the underlying cause.
How Expanded MiDOG Testing Fits into the Clinical Workflow.
Start broad. Add depth when the case demands it.
MiDOG All-in-One Essential Test
Your report includes:
• Detection of clinically relevant bacteria and fungi
• Genotypic antimicrobial resistance markers for identified bacterial pathogens
• Supplemental context comparing results to MiDOG’s reference database
MiDOG Expanded Testing & Advanced Add-Ons
When additional diagnostic clarity is needed, expanded testing and optional add-on modules provide deeper insight into complex or unresolved cases, including organisms or biological factors that may influence treatment response.
Expanded insight may include:
• Detection of parasitic organisms
• Biofilm-associated markers linked to persistent infection
• Toxin-associated markers that support clinical interpretation
• Additional genomic context to guide next-step decision-making
The MiDOG Workflow
How to Order Expanded MiDOG Testing (No Workflow Disruption)
- Order through the MiDOG Portal
- Select your testing option: All-in-One Essential Test or Essential Test with Advanced Add-On Modules
- Collect the sample following standard submission guidelines for the selected sample type
- Submit as usual using the same packaging and shipping workflow
- Receive your report through your standard results workflow
Workflow note: Add-ons may be selected at sample submission or added after the core report is delivered.
Collect. Seal. Ship. Diagnose.
Fast, simple, and reliable for any species. This is how easy it is to collect your sample and ship it to us.
Collect
Use the sterile swab or urine collection materials provided in your kit.
Secure
Transfer your sample into the provided MiDOG tube, close tightly, and complete the test requisition form.
Ship
Place everything into the prepaid return mailer and send it back to our lab. U.S. shipping is free both ways.
Receive Results
Receive your full NGS report in 2-3 days of sample arrival
Expected turnaround time (TAT)
Typical TAT: 48 hours from lab receipt. Most reports are delivered within this window; sample quality, complexity, or reprocessing/QC may extend timing.
Sample types
Compatible with all Animal Species and Sample Types:
Nasal, Skin, Oral, Wound, and Ear Swabs, Urine, Feces, Blood, Bile, Joint Fluid, BAL, Eyes/Nasal Washes, Tissue, Nail Clippings, and so much more.
Have a case that isn’t resolving?
MiDOG Expanded Testing can help identify parasites, biofilm-associated markers, toxin signals, and complex polymicrobial infections.
Frequently Asked Questions
Please contact us through our contact page, and one of our consultants will respond as soon as possible.
What is MiDOG All-in-One Extended Testing
With the official launch of the MiDOG All-in-One test, we are expanding our testing capabilities beyond amplicon-based 16S/ITS analysis. This new approach allows for the detection of:
- Additional pathogens not previously covered (Protozoan, Nematode, and Flatworm parasites)
- Bacterial biofilm-related genes, which may affect infection persistence and treatment response
- Bacterial virulence and toxin genes that can impact clinical outcomes
What new types of pathogens will be detectable?
- The new MiDOG All-in-One test will detect all Eukaryotic parasite species down to the species level and all bacterial and fungal pathogens in our previous service. The new pathogens will include:
- All Protozoan parasite species (including but not limited to: Entamoeba, Giarda, Cystoisospora, Eimeria, Cryptosporidium, Toxoplasma, Plasmodium, and Tritrichomonas)
- All Hookworm, roundworm, whipworm, and tapeworm species (including but not limited to: Dirofilaria, Toxocara, Baylisascaris, Trichuris, Diplidium, Echinococcus, Echinostoma, Taenia, Fasciola, Leishmania, Opisthorchis, Rallietina, Syphaci, Trichinella
All Oomycete parasite species (including but not limited to: Pythium, Saprolegnia, Aphanomyces, Halioticida)
What are Biofilms?
A biofilm is a community of bacteria that attach to surfaces and produce a protective matrix. In clinical contexts, biofilms can make infections more difficult to treat because they:
- Contribute to antibiotic resistance by creating microenvironments where bacteria can survive higher drug concentrations
- Facilitate chronic or persistent infections
- Increase the risk of device-related infections (catheter and surgical implants)
- Promote recurrent urinary tract infections (UTIs) by allowing bacteria to persist on the bladder surfaces
- Impair wound healing by creating a persistent source of infection
- Contribute to chronic or non-healing wounds, such as diabetic foot ulcers or pressure sores
The MiDOG All-in-One test can detect bacterial genes associated with biofilm formation for each individual case, providing clinicians with actionable insights that were previously not accessible.
Which Virulence/toxin genes will MiDOG All-in-One test for?
Toxins and virulence genes are the weapons of bacterial pathogens, allowing them to initiate disease and cause clinical signs associated with illness. Toxin/virulence genes can help guide clinical decisions by helping to signal the bacterial pathogens of concern in each case. The MiDOG All-in-One will detect over 120 families of bacterial toxin genes including:
- Enterotoxins and Shiga toxins (Shigella and pathogenic coli strains)
- Anthrax toxin (Bacillus anthracis)
- Tetanus toxin (Clostridium tetani)
- Diphtheria toxin (Corynebacterium)
- Botulinum toxins (Clostridium botulinum)
- Pertussis toxins (Bordetella pertussis)
- Typhoid toxins (Salmonella)
- Hemolysins – toxins possessed by several pathogen species that allow them to cleave red blood cells and other host cells (Rickettsia, Streptomyces, Actinobacillus, Aeromonas, UTI, and septic E. coli pathogen strains)
What major groups of Toxin families can the All-in-One Test screen for?
- Enterotoxins
- Food poisoning and other GI tract issues
- Exotoxins
- Toxins that the bacteria excrete to poison the host and surrounding environment
- Hemolysins/Lysins
- Toxins that bacteria produce to cut open red blood cells and other cells to feed off the host.
- Endotoxins
- Toxins that bacteria produce internally that are stored in the bacterial cells and released when they die.
- Can cause toxic shock syndrome if too many endotoxin-producing bacteria are killed at once.
Expanded Testing Lead Capture
If you’re managing a complex or unresolved infection, our team can help determine whether Expanded Testing or targeted add-ons may provide additional diagnostic insight.